Can be used in conjunction with routine cervical/endocervical
smears in individuals with a uterus or alone in patients with
Specimens required: Lateral vaginal wall smear or smear of sample
from a clinically concerning area.
Supplies: Vaginal speculum, one or more plastic cervical
spatulas, fixative** (spray fixative or 95% ethanol), one or
more clean glass slides (single-end frosted), black lead pencil
lab pencil and test request form.
Pap kits are available from PLA.
a) If only a routine lateral vaginal wall sample will be obtained,
label one slide with patients first and last name, and source
of specimen. If vaginal sample will be obtained in conjunction
with a cervical and endocervical component, make sure that the
slides are also appropriately labeled according to site.
b) If smears from separate vaginal areas are also to be obtained,
label the sites accordingly as to specific site (e.g. left lateral
vaginal wall, posterior vaginal wall, etc.).
c) Obtain specimen prior to bimanual evaluation. Use an un-lubricated
speculum (saline or warm water may be used).
d) Scrape the desired region of the vaginal mucosa with the spatula.
e) Withdraw the spatula and spread the material quickly and evenly
onto the glass slide (if cervical/endocervical smears are also
obtained, or smears from other areas of the vagina are obtained,
make certain that each smear is performed on the corresponding
f) Fix immediately, (spray with fixative holding the spray bottle
approximately 10-12 inches from the slide or drop slide into
95% ethanol fixative).
g) Complete the Cytology test request form, including relevant
clinical information. Submit the specimen to the Cytopathology
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