Indications: Evaluation of potential changes
associated with in utero DES exposure.
Specimens required: Lateral vaginal wall sample, cervical sample,
Supplies: Vaginal speculum, one or more cervical spatulas,
one extended-tip spatula, cytobrush, fixative (spray fixative
or 95% ethanol), two or more clean glass slides (single end frosted),
black lead lab pencil, and test request form. Pap kits are available
a) Label two slides with patients first and last name,
date of birth and specimen source.
b) Label one slide C and one LVW. Label
others as necessary (see below).
c) Obtain specimens prior to bimanual examination. Use an un-lubricated
speculum (saline or warm water may be used). After visualization
of the upper 1/3 of the vagina is accomplished use the spatula
to scrape the upper 1/3 of either lateral vaginal wall. Withdraw
the spatula and spread the material quickly and evenly onto the
slide clearly marked LVW.
d) Fix immediately (drop slide into fixative or spray with fixative
holding the spray bottle approximately 10-12 inches from the
e) Scrape additional areas of the vagina that appear abnormal.
Label slides accordingly (prior to sampling) and spread and fix
f) Obtain routine cervical and endocervical specimens as outlined
in the respective procedures. These smears should be collected
on the slide labeled C. Complete the Cytology test request form, including relevant
history. Submit the specimen to the Cytopathology Laboratory.
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