Histopathology / Cytopathology / Hematopathology / PLA Home Page
Test indication: For detection and characterization of endoscopically visible gastrointestinal lesions; for the identification of some microbiologic pathogens (e.g. herpes, CMV, and Candida, etc.).
Specimen types: Brushings (Esophageal, GI Junction, Gastric, Duodenal, Bile Duct, Other).
Routine cytopathologic evaluation.
Specimen requirement: Endoscopically-directed brushing sample of the identified lesion.
Supplies: Standard endoscopy equipment. One (or more if necessary) 5 to 10 mL vial or tube of sterile normal saline or fixative (either CytoRich Red cytology preservative or 50% ethanol).
Collection procedure: Instruct the patient to fast overnight or for a minimum of six hours prior to the procedure. Identify the lesion in question and obtain a brushing sample of the lesion using standard endoscopy technique.
It is important to brush the edges of an ulcer, as well as the
floor, in order to obtain diagnostic material. Upon withdrawing
the brush, agitate the brush vigorously in a 5 to 10 mL vial
of saline or fixative. DO NOT APPLY THE BRUSH DIRECTLY TO SLIDES.
If possible, detach the brush and leave in the vial. Label the
vial with the patients first and last name and specimen
source. Submit the specimen along with the completed
Cytology test request form to the Cytopathology Laboratory.
Fixation: Preferred specimen: Fresh, no fixative. If transport
will be delayed more than 4 hours the specimen should be partially
fixed by adding a equal amount of 50% ethanol or CytoRich Red
cytology preservative to the specimen or submit the specimen
in a proprietary transport medium used for liquid based systems
supplied by the laboratory (e.g. Cyto-Rich Red, CytoLyt)
Causes for rejection: Incomplete patient requisitions, unlabeled/incorrectly labeled specimen, frozen specimen, incorrect test ordered for specimen, specimen in incorrect or expired fixative, specimen from unauthorized person.
Specimen stability: 4 hours fresh refrigerated (4°C or 39°F), all GI specimens tend to deteriorate rapidly in the fresh state due to enzymatic activity endogenous to the GI tract.
CPT Code(s): 88104