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Oral Scraping

Test indications: Detection and characterization of malignancy and infectious processes in the oral cavity.

Methodology: Routine cytopathology evaluation.
Performed: Monday-Friday.
Reported:1 day (unless special studies are required).

Specimen type: Oral Scrapings.

Specimen required: Direct smear of material collected from the oral mucosa.

Supplies: Two (or more) clean glass slides, fixative (95% ethanol) or spray fixative, oral scraping spatula, lead pencil, biohazard transport bag and test request form

Collection procedure: Label the frosted end of the slides or vial with the patient’s first and last name and specimen source in pencil. If slides are used, place in a container filled with 95% ethanol so that the slides are completely covered. Gently scrape the area of the abnormality. Remove one of the slides from the fixative. Quickly and evenly smear the collected material on one of the glass slides. Immediately re-immerse the slide in fixative. Repeat the process with the second slide if necessary for better diagnostic yield. Submit the specimen and the completed test request form to the Cytopathology Laboratory.

Fixation immersion: Place the smear(s) into 95% ethanol. If the specimen is immersed in alcohol, it may remain in the alcohol for transport for to the laboratory or alternatively the specimen can be immersed in alcohol for 20-30 minutes, removed and allowed to air dry then place in a container/mailer to be transported to the laboratory.

Spray fixation: If spray fixed only quality controlled cytology fixative should be used. The manufacture’s instructions should be followed. Hold spray 6-10 inches (15-25 centimeters) from the glass slide when applied.

Transport: Fixed smears should be submitted in slide mailer(s), clearly labeled with patient name and specimen site(s). Slides in fixative should be submitted in leak proof container(s) that protect against breakage and clearly labeled with patient name and specimen site(s). Paper requisitions that accompany the specimen or slides in fixative should be placed in the outside pocket of the biohazard bag to avoid exposure to any leakage of fixative. Submit the specimen and the completed test request form to the Cytopathology Laboratory.

Causes for rejection: Incomplete patient requisitions, unlabeled/incorrectly labeled specimen, frozen specimen, incorrect test ordered for specimen, specimen submitted in incorrect or expired fixative, specimen from unauthorized person.

Specimen stability: Fixed slides: Indefinitely.

Reference interval: By report.

CPT Code(s): Depending on collection method: 88108 Concentration and interpretation or 88112 Selective cellular enhancement with interpretation or 88104 Smears with interpretation. Additional CPT codes may apply if special studies are required.

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