Histopathology / Cytopathology / Hematopathology / PLA Home Page
Indications: Detection of suspected malignancy utilizing lavage and brushing specimens obtained cystoscopically bladder washing, renal pelvis washing/brushing, ureteral washing/brushing or urethral washing); staging of urologic malignancies.
Specimen required: 10 ML (or more) of an appropriately-collected, cystoscopically-derived specimen.
Supplies: Standard cystoscopy equipment. Clean collection container of appropriate size. Fixative (50% ethanol).
Washing: Using standard cystoscopy technique, obtained washing specimens, carefully denoting specific specimen sites for each specimen on the test request form. Submit the specimen fresh to the Cytopathology Laboratory along with the completed Cytology test request form. If transport of the specimen will be delayed more than 24 hours, add an equal volume of 50% ethanol alcohol. If transport time will be less than 24 hours, or fixative is not available, the specimen should be refrigerated or kept on wet ice until transport to the lab.
Brushing: Using standard cystoscopy technique, identify the lesion in question and obtain a brushing sample of the lesion.
Note: It is important to brush the edges of an ulcer, as well as the floor, in order ot obtain diagnostic material. Upon withdrawing the brush, agitate the brush vigorously in a 5 to 10 mL vial of saline or fixative. DO NOT APPLY THE BRUSH DIRECTLY TO SLIDES. If possible, detach the brush and leave it in the vial. Label the vial with the patients first and last name, date of birth and specimen source. Submit the specimen along with the completed Cytology test request form to the Cytopathology laboratory.
If transport of the specimen will be delayed
more than four hours, the specimen should be submitted in CytoRich
Red cytology preservative or 50% ethanol. If transport
time will be less than four hours, or fixative is not available,
the specimen should be refrigerated or kept on wet ice until
transport to the lab.