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Urine (Void or Catheterized)

Test indication: Detection and characterization of malignant cells and other urologic abnormalities in symptomatic (usually hematuria) patients; screening for malignancy in selected individuals at high risk for the development of urologic malignancy; detection and characterization of some non-neoplastic renal diseases in symptomatic (usually hematuria) patients.

Methodology: Routine cytopathologic evaluation.
Performed: Monday-Friday.
TAT: 1 day.

Specimen type: Voided/Catheterized urine.

Special required: 50 mL of an appropriately collected voided or catheterized urine specimen. Early morning and 24 hour samples are not recommended for cytologic evaluation.

Supplies: Clean collection container of appropriate size. Standard catheterization equipment (for catheterized urine). Fixative (50% ethanol).

Collection procedure: For purposes of obtaining the greatest yield of diagnostic material, a second-morning voided urine specimen should be obtained, if possible.

A midstream, clean-catch specimen is recommend to avoid vaginal contamination in female patients. If the patient must be catheterized to obtain the specimen, this should be noted on the test request form as catheterization can lead to artifacts which may be misinterpreted without the knowledge that the specimen was catheterized. Label the vial with the patient’s first and last name, date of birth, and specimen source. Submit the specimen to the Cytopathology Laboratory along with the competed Cytology test request form.

Fixation: If transport of the specimen will be delayed more than 24 hours, add an equal volume of 50%-70% ethanol (if sample size is too large to accommodate this volume, a well mixed aliquot (50 mL) of the specimen with an equal volume of fixative may be utilized). If transport time will be less than 24 hours, or fixative is not available, the specimen should be refrigerated or kept on wet ice until transport to the lab.

Transport: Each specimen should be placed in a clearly labeled container that is leak proof. Place specimen inside a biohazard transport bag. Paper requisition(s) that accompany the specimen or slides in fixative should be placed in the outside pocket of the biohazard bag to avoid exposure to any leakage of fixative.

Causes for rejection: Incomplete patient requisitions, unlabeled/incorrectly labeled specimen, frozen specimen, incorrect test ordered for specimen, specimen in incorrect or expired fixative, specimen from unauthorized person.

Stability: Ambient temperature up to 24 hour fresh, refrigerated 72 hours add fixative 50-70% ethanol.Reference interval: By report.

CPT code(s): 88108 Concentration and interpretation or 88112 Selective cellular enhancement with interpretation and/or 88106 Cytopathology, filter. Additional CPT codes may apply if special studies are required.

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