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Washings

Test indication: The detection and characterization of ill-defined or invisible premalignant/malignant cells.

Specimen required: Washings can be collected from varies body sites including but not limited to the following sites: Respiratory tract (bronchial washings), Abdominal/Pelvic washings, Gastrointestinal tract, Larynx, Nasopharynx, Oral Cavity, Paranasal sinus, Prostate, Renal/Ureteral washing.

Methodology: Routine cytopathology evaluation.
Performed: Monday-Friday.
Reported: 1 day.

Specimen requirement: 10 mL-50 mL aliquots of balanced saline solution washed over a directly visualized area. Preferable submit sample unfixed to the laboratory in clean container labeled with patients name and source of specimen accompanied by a patient requisition with pertinent clinical information. If the specimen cannot be delivered to the laboratory immediately then a fixative should be added to the sample.

Supplies: Clean container, patient requisition, fixative (if specimen cannot to sent to lab immediately) and biohazard specimen bag.

Collection procedure: Using standard technique and equipment locate the suspect area and rinse small aliquots directly over visualized area and remove immediately with suction and place in clean container, submit unfixed to the laboratory. If a delay is expected, then sample may be partially fixed in 50% ethanol equal to the volume of the specimen, or in a proprietary transport medium supplied by the lab. Any added fixative should be noted on the requisition.

Fixation: Preferable, fresh unfixed sample. If transport time to the lab will be less than 24 hours, or fixative is not available, the specimen should be refrigerated or kept on wet ice until transport to the lab. If a longer delay (greater than 24 hours) is expected than the sample may be partially fixed in 50% ethanol equal to the volume of the specimen, or in a proprietary transport medium supplied by the lab. Any added fixative should be noted on the requisition.

Transport: Each specimen should be placed in a clearly labeled container that is leak proof. Place specimen in a biohazardous transport bag. The paper requisition(s) that accompanies the specimen should be place in the outside pocket of the biohazard bag to avoid exposure to any leakage.

Causes for rejection: Incomplete patient requisitions, unlabeled/incorrectly labeled specimen, frozen specimen, incorrect test ordered for specimen, specimen submitted in incorrect or expired fixative, specimen from unauthorized person.

Specimen stability: Up to 72 hours for a fresh specimen, no fixative added and refrigerated (4°C) or (39°F). Fixative added to specimen at room temperature indefinitely.

Reference interval: By report.

CPT code(s): 88108 Concentration and interpretation; 88305 Cell block. Additional CPT codes may apply if special studies are required.

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